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KMID : 0380020090240010106
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 1 p.106 ~ p.112
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On-line monitoring of microorganism cultivation processes using optical sensing membranes for simultaneous detection of dissolved oxygen and pH
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Kim Chun-Kwang
Lee Jong-Il
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Abstract
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An optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen(DO) and pH value simultaneously. It has employed HPTS as a pH sensitive dye and a ruthenium(II) complex as a DO sensitive dye. The sensing membrane has been applied to wells in a 24-well microtiter plate. Using the 24-well microtiter plate the concentrations of dissolved oxygen and pH values have been on-line monitored during the cultivations of E.coli DH5, B.cereus 318 and P.pastoris X-33. On-line monitoring of DO and pH in microorganism cultivation processes showed good performance of the sensing membrane containing 5 mM HPTS and 2 or 5 mg/mL Rudpp.
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KEYWORD
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Process monitoring, Optical sensing membrane, DO, pH, Microorganism
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